Fingerprinting of sorghum disease nursery cultivars by random amplified polymorphic DNA markers. / M. Qhobela

Public ISBD Accompagne Drought-tolerant crops for Southern Africa Titre : Fingerprinting of sorghum disease nursery cultivars by random amplified polymorphic DNA markers. Type de document : texte imprimé Auteurs : M. Qhobela, Auteur Editeur : ICRISAT Année de publication : 1996 Importance : p 211-218 Langues : Anglais (eng) Catégories : SCIENCES AGRICOLES Mots-clés : MALADIE DE SORGHO  RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS Résumé : Random Amplified Polymorphic DNA (RAPD)markers were used to characterize sorghum cultivars in the SADC/ICRISAT disease nursery. The use of single primers, 10 nucleotides in length and of arbitrary nucleotide sequence resulted in the amplification of DNA fragments that were unique to some cultivars in the disease nursery. Fingerprint images of 10 cultivars are presented and the application of RAPD fingerprinting for the identification of cultivars, the general evaluation of germplasm and the possible improvement of the sorghum breeding program are discussed . It is concluded that genetic marker-based technology can be used to assist researchers in future sorghum breeding work. Numéro du document : A10433/F00 132 Niveau Bibliographique : 2 Bull1 (Theme principale) : SCIENCES ET PRODUCTION VEGETALES Bull2 (Theme secondaire) : SYSTEMES ET MODES DE CULTURE Accompagne Drought-tolerant crops for Southern Africa Fingerprinting of sorghum disease nursery cultivars by random amplified polymorphic DNA markers. [texte imprimé] / M. Qhobela, Auteur . - ICRISAT, 1996 . - p 211-218. Langues : Anglais (eng) Catégories : SCIENCES AGRICOLES Mots-clés : MALADIE DE SORGHO  RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS Résumé : Random Amplified Polymorphic DNA (RAPD)markers were used to characterize sorghum cultivars in the SADC/ICRISAT disease nursery. The use of single primers, 10 nucleotides in length and of arbitrary nucleotide sequence resulted in the amplification of DNA fragments that were unique to some cultivars in the disease nursery. Fingerprint images of 10 cultivars are presented and the application of RAPD fingerprinting for the identification of cultivars, the general evaluation of germplasm and the possible improvement of the sorghum breeding program are discussed . It is concluded that genetic marker-based technology can be used to assist researchers in future sorghum breeding work. Numéro du document : A10433/F00 132 Niveau Bibliographique : 2 Bull1 (Theme principale) : SCIENCES ET PRODUCTION VEGETALES Bull2 (Theme secondaire) : SYSTEMES ET MODES DE CULTURE

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